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. 2021 May 29;12(6):558. doi: 10.1038/s41419-021-03828-z

Fig. 3. Cell spreading is impaired by MDM4 in a 3D-bioprinting assay.

Fig. 3

a Rendering of the 3D-bioprinting geometry used. b Representative pictures of bioprinted constructs carrying MCTS and HUVEC at day 0 (upper panels, black arrow points to the MCTS) and day 20 after bioprinting (lower panels); red and green arrows point to cancer cells spreading and to vessels-like structures, respectively. Right panels show the rendering of day 0 bioprinting strategy (upper panel) and cell invasion at day 20 (lower panel); scale bars = 200 mM. c Confocal microscopy of constructs showing the vessels-like structures (stained with sheep anti-Von Willebrand Factor AbCam Cat# ab11713, green signal), cancer cells expressing mCherry (red signal), and MDM4 (stained with Mouse anti-MDM4 Ab OriGene Cat# TA505706, yellow signal), DAPI stains nuclei. White arrows point to migrating cancer cells. The drawing shows the rendering of cancer cells escaping from the MCTS and entering vessels-like structures.