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. 2021 May 29;12(6):558. doi: 10.1038/s41419-021-03828-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Pathway analysis of mTOR signaling in SK-OV-3 cells. Red and green colors underlie proteins differently expressed in MDM4- compared with Empty vector-SK-OV-3 cells (red = upregulated, green = downregulated proteins). b WB of Empty Vector or MDM4-SK-OV-3 cells showing S6K1 and p70S6K1^T389 expression (left panel) and quantification (right panel) (n = 3, one-sample t test p < 0.001). c WB of indicated proteins in tumor nodules (one per mouse) generated by IP injection of Empty Vector or MDM4-SK-OV-3 cells in mice; lower panels show quantification (n = 7 mice, two-sided Student’s t test, ***p < 0.001, *p = 0.048). d SunSet assay of Empty Vector- and MDM4-SK-OV-3 cells treated with rapamycin for 6 hours; quantification of two independent assays (right panel). e WB of indicated proteins in whole-cell extract (WCE) and co-immunocomplex from SK-OV-3 cells grown in EBSS for 1 hour and then in absence or presence of complete medium for additional 1 hour (CM). 1 mg of WCE was immunoprecipitated with anti-MDM4 antibody (IPαMDM4) or control Ig (IPCTL). Right panel shows the analysis of 1/100 of WCE.