Fig. 3.

Locations of ANTAR-target RNAs within their genomic context. a Schematic shows the location of ANTAR-target RNAs. RNAs are grouped in three categories- intergenic’ for RNAs that lie at a distance> 15 nt from start of ORF, ‘sequester RBS or AUG’ for RNAs which overlap with the ribosome binding site or start codon and ‘inside ORF’ for RNAs which lie after the start codon. 10-nucleotide flanking regions on either side of the dual stem loop structure are included in the distance calculations. b Histogram shows distribution of RNAs versus their distance from the respective ORF. Several RNAs are found near the ORF start site, sequestering either RBS or AUG (yellow). c Plot shows total number of predicted RNAs in three categories as described in panel A. 47 RNAs (dashed brown box) in the ‘sequester RBS or AUG’ category and 15 RNAs (dashed red box) in the ‘inside ORF category were assigned based on alternate ORF predictions. d Representative RNAs from ‘sequester RBS or AUG’ category are shown with the ANTAR-target RNA structure marked. Potential RBS (red) and start codon (yellow) are shown. Genomic context of these RNAs (blue) are shown with ORFs (purple) with their NCBI gene annotations. E Representative RNAs from the ‘inside ORF’ category are shown. The dual stems of the ANTAR-target RNA are highlighted in pink and blue. Start codon is marked in yellow