Fig. 5.

Knockdown of SIRT1 enhanced OGD/R-induced cell death via FoxO3. a Relative SIRT1 protein level in cells transfected with sh-NC or sh-SIRT1. b MTT assay to analyze cell viability in transfected cells following OGD/R. c TUNEL staining to measure the number of apoptotic cells in transfected cells upon OGD/R. d Relative protein levels of SIRT1, FoxO3, SPHK1, and NF-κB p65 in transfected cells following OGD/R. e Relative levels of TNF-α, IL-6, and IL-1β in transfected cells following OGD/R. All the results were shown as mean ± SD (n = 3), which were three different experiments performed in triplicate. *P < 0.05, **P < 0.01, ***P < 0.01