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miR-19a/b-3p directly targeted SIRT1. a Predicted binding sites between miR-19a/b-3p and SIRT1 mRNA and the sequences of SIRT1 3′UTR MUT. b Relative luciferase activities of WT-SIRT1 3′UTR and MUT-SIRT1 3′UTR in cells transfected with mimics NC or miR-19a/b-3p mimics. c RIP to analyze the binding between WT-SIRT1 3′UTR and miR-19a/b-3p. All the results were shown as mean ± SD (n = 3), which were three different experiments performed in triplicate. *P < 0.05, **P < 0.01, ***P < 0.01
