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. 2021 May 29;21:284. doi: 10.1186/s12935-021-01993-x

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — LncRNA WDFY3-AS2 upregulated SDC4 expression by sponging miR-139-5p in OC A2780-DDP cells. A Dual luciferase reporter assay validated miR-139-5p binding to SDC4. B RNA pull-down confirmed that the binding of miR-139-5p and SDC4 by the miR-139-5p-biotin. C qRT-PCR expression analysis of SDC4 in OC A2780-DDP cells. D Western blotting assessment of SDC4 protein expression in OC A2780-DDP cells. E The negative correlation between miR-139-5p and SDC4, n = 15. F, G, The mRNA and protein expression of SDC4 in si-WDFY3-AS2 transfected cells with co-transfection of miR-139-5p inhibitor. ^###P < 0.001 vs. si-WDFY3-AS2 + inhibitor NC, ***P < 0.001 vs. control