Skip to main content
. 2021 Apr 26;27(6):gaab031. doi: 10.1093/molehr/gaab031

Figure 1.

Figure 1.

The combination of GDF9 and BMP15 strongly stimulates SFRP4 expression in primary human cumulus cells. (A) Primary human cumulus cells (CCs) were treated for 48 h with vehicle (C), GDF9 (G: 5 ng/ml), BMP15 (B: 5 ng/ml), GDF9 plus BMP15 combined (GB: 5 ng/ml each) in the presence or absence of FSH (50 ng/ml). SFRP4 mRNA levels were determined by qPCR and expressed relative to Rpl19. Columns represent the mean ± SEM. Columns with different letters differ significantly by one-way ANOVA analysis with repeated measures and Bonferroni correction, a–b and b–c P < 0.05, a-c P < 0.01 (n = 9). (B) Primary human CCs were treated for 48 h with vehicle (C), GB: 0.1, 0.6, 2 or 5 ng/ml G and B each, in the presence or absence of FSH (50 ng/ml). SFRP4 mRNA levels were determined by qPCR and expressed relative to Rpl19. Columns represent the mean ± SEM. Columns with different letters differ significantly by one-way ANOVA analysis with Bonferroni correction, a–b, d–e, c–e, a–c P < 0.05, and a–e P < 0.01 (n = 9).