Figure 2.

The combination of GDF9 and BMP15 stimulates SFRP4 protein synthesis. Cumulus cells were obtained from four patients. For Patient no. 128, cells were treated with GDF9 (G: 5 ng/ml), BMP15 (B: 5 ng/ml) or GDF9 and BMP15 combined (GB: 5 ng/ml each). The cells from the other patients (#309, #312, #132) were treated with vehicle or GB (5 ng/ml each). SFRP4 and β-actin protein levels were quantified by the western blot. The bands corresponding to the vehicle or GDF9 plus BMP15-treated cells were quantified for all patients, plotted in the inserted graph where columns represent the mean ± SEM, and analysed using paired t-test, (***) P < 0.01 (n = 4). Full blot images are presented in Supplementary figure S1.