Figure 4.

Treatment with GDF9 and BMP15 decreases β-catenin/TCF activity. (A) Cells were infected with lentivirus carrying the β-catenin/TCF-Luc reporter and incubated overnight prior to 48 hours treatment with vehicle (C) or GDF9 and BMP15 (GB: 5 ng/ml each). Luciferase activity was quantified and expressed relative to the control. Columns represent the mean ± SEM and analysed using paired t-test, P < 0.05 (n = 4). (B) Cells were processed as in A but treated with FSH (50ng/ml) or FSH plus GDF9 plus BMP15 (GB). Columns represent the mean ± SEM, columns with different letters differ significantly by one-way ANOVA with Bonferroni correction, a–b P < 0.05, a–c P < 0.01, b–c P < 0.05 (n = 4).