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. Author manuscript; available in PMC: 2021 May 30.
Published in final edited form as: Cell Syst. 2020 Dec 7;12(1):41–55.e11. doi: 10.1016/j.cels.2020.11.002

Figure 1. Generation and Assessment of Fetal Liver Organoid (FeLO).

Figure 1.

(A) Schematic of FeLO generation process from GATA6-engineered hiPSC. Full culture details are listed in the STAR Methods section.

(B) Heatmap showing the CellNet classification scores of FeLO for listed tissues or cell types before induction of GATA6 (day 0) and at day 5, 10, and 17 of culture.

(C) Liver NIS analysis using CellNet reveals transcriptional regulators that show deficiency (negative score) in day 17 FeLO compared with the human liver training set (n = 2).

(D–G) qPCR data for transcription factor screening in FeLO, increase in ALB following PROX1 induction (D), synergistic activation of G6PC with PROX1 and ATF5 (E), modest increase in CYP3A4 without SynTF(CYP3A4) (F), and significant CYP3A4 upregulation with SynTF(CYP3A4) and synergistic effect of PROX1 and ATF5 co-expression on CYP3A4 activation (G). Reference sample is untransduced. #p < 0.05, ##p < 0.01 over each other condition, *p < 0.05, ****p < 0.0001 (n = 3 except n = 6 for CYP3A4 only in L). Significance was determined by one-way ANOVA with Tukey’s multiple comparison test.

Data are represented as mean ± SEM for (D–G).

See also Figure S1.