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. Author manuscript; available in PMC: 2022 Jun 1.
Published in final edited form as: J Immunol. 2021 May 10;206(11):2503–2507. doi: 10.4049/jimmunol.2100195

Table I.

Amino acid sequences for peptides used in in vitro cytokine stimulation assays.

Spike protein aa# Sequence
1–14, 319 MFVFLVLLPLVSSQR
5–14, 319–323 LVLLPLVSSQRVQPT
9–14, 319–327 PLVSSQRVQPTESIV
14, 323–331 SQRVQPTESIVRFPN
321–335 QPTESIVRFPNITNL
325–339 SIVRFPNITNLCPFG
329–343 FPNITNLCPFGEVFN
333–347 TNLCPFGEVFNATRF
337–351 PFGEVFNATRFASVY
341–355 VFNATRFASVYAWNR
345–359 TRFASVYAWNRKRIS
349–363 SVYAWNRKRISNCVA
353–367 WNRKRISNCVADYSV
357–371 RISNCVADYSVLYNS
361–375 CVADYSVLYNSASFS
365–379 YSVLYNSASFSTFKC
369–383 YNSASFSTFKCYGVS
373–387 SFSTFKCYGVSPTKL
377–391 FKCYGVSPTKLNDLC
381–395 GVSPTKLNDLCFTNV
385–399 TKLNDLCFTNVYADS
389–403 DLCFTNVYADSFVIR
393–407 TNVYADSFVIRGDEV
397–411 ADSFVIRGDEVRQIA
401–415 VIRGDEVRQIAPGQT
405–419 DEVRQIAPGQTGKIA
409–423 QIAPGQTGKIADYNY
413–427 GQTGKIADYNYKLPD
417–431 KIADYNYKLPDDFTG
421–435 YNYKLPDDFTGCVIA
425–439 LPDDFTGCVIAWNSN
429–443 FTGCVIAWNSNNLDS
433–447 VIAWNSNNLDSKVGG
437–451 NSNNLDSKVGGNYNY
441–455 LDSKVGGNYNYLYRL
445–459 VGGNYNYLYRLFRKS
449–463 YNYLYRLFRKSNLKP
453–467 YRLFRKSNLKPFERD
457–471 RKSNLKPFERDISTE
461–475 LKPFERDISTEIYQA
465–479 ERDISTEIYQAGSTP
469–483 STEIYQAGSTPCNGV
473–487 YQAGSTPCNGVEGFN
477–491 STPCNGVEGFNCYFP
481–495 NGVEGFNCYFPLQSY
485–499 GFNCYFPLQSYGFQP
489–503 YFPLQSYGFQPTNGV
493–507 QSYGFQPTNGVGYQP
497–511 FQPTNGVGYQPYRVV
501–515 NGVGYQPYRVVVLSF
505–519 YQPYRVVVLSFELLH
509–523 RVVVLSFELLHAPAT
513–527 LSFELLHAPATVCGP
517–531 LLHAPATVCGPKKST
521–535 PATVCGPKKSTNLVK
525–539 CGPKKSTNLVKNKCV
529–541, 2xH KSTNLVKNKCVNFHH
533–541, 6xH LVKNKCVNFHHHHHH
HSVgB 498–505 SSIEFARL
511*–518 VVLSFELL