Table 2.
Crystal Structures of RUVBL1, RUVBL2 and RUVBL1/2 complex
| PDB Code | Methods (Å) | Macromolecules | Ligands | Organisms | References |
|---|---|---|---|---|---|
| 2C9O | X-ray (2.2) | RUVBL1: K9-C141, H156-N247, G277-D449 (Sequence: M1-K456) | ADP | hsa | [80] |
| 2CQA | NMR (NA) | RUVBL2 C-terminal: GSSGSSG+K132-R213+SGPSSG (Sequence: GSSGSSG+K132-R213+SGPSSG) | - | hsa | - |
| 2XSZ | X-ray (3.0) | RUVBL1 ΔDII: E5-R123, I235-R249, T269-K453 (Sequence: MVHHHHHHLLVPRGS+K2-E126+GPPG+I234-K456)/RUVBL2 ΔDII: I22-I131, H240-T254, E267-K456 (Sequence: MDYKDDDDKENLYFQG+A2-E133+GPPG+V238-S463) | ATP | hsa | [81] |
| 3UK6* | X-ray (3.0) | RUVBL2 ΔDII: T8-E12, D15-I131, T241-F261, E267-K444 (Sequence: M1-K132+AGA+V239-S463+LEHHHHHH) | ADP | hsa | [82] |
| 4WVY* | X-ray (3.6) | RUVBL1: I4-E142, T157-P251, I257-T449 (Sequence: M1-S462)/RUVBL2: H26-I145, Q157-A209,F223-D457 (Sequence: MGS SHHHHHHHHSSGLEVLFQGPGS+M1-S488) | ATP | cthr | [83] |
| 4WW4* | X-ray (2.9) | RUVBL1: Q3-V136, S156-K169, L173-D174, Q182-C201, R206-P251, D256-T451, G455-H458 (Sequence: M1-S462)/RUVBL2: T7-R149, G153-R212, F223-G229, Q232-V456 (Sequence: MGSSHHHHHHHHSSGLEVLFQGPGS+M1-S488) | ADP | cthr | [83] |
| 5FM6* | X-ray (3.0) | RUVBL1: Q3-T140, T157-T198, G205-S450 (Sequence: GA+M1-S462)/RUVBL2: S8-E14, H26-R149, G158-R210, T221-V455 (Sequence: GA+M1-S488) | ADP | cthr | [84] |
| 5FM7* | X-ray (2.9) | RUVBL1: Q3-E138, I160-T198, G205-P251, Q255-S450 (Sequence: GA+M1-S462)/RUVBL2: S8-L15, I21-R149, K159-A209, K222-V455 (Sequence: GA+M1-S488) | ADP | cthr | [84] |
| 5OAF | EM (4.1) | INO80 complex RUVBL1: K11-D452 (Sequence: M1-K456)/RUVBL2: V16-R211, P228-A449 (Sequence: M1-S463) | ADP | hsa | [85] |
| 5OUN | NMR (NA) | RUVBL2 DII: GPHM+E132-T234 (Sequence: E132-T234) | - | sce | [86] |
| 6FHS | EM (3.8) | INO80 complex RUVBL1: I4-S462 (Sequence: M1-S462)/RUVBL2: L20-F458 (Sequence: M1-S488) | ADP | cthr | [87] |
| 6FML | EM (4.3) | INO80 complex RUVBL1: I4-S462 (Sequence: M1-S462)/RUVBL2: L20-F458 (Sequence: M1-S488) | ADP | cthr | [87] |
| 6FO1 | EM (3.6) | R2TP Subcomplex RUVBL1: E5-I124, I235-R249, T269-K456 (Sequence: M1-K456)/RUVBL2: I22-I131, H240-R253, E267-N453 (Sequence: M1-S463) | ADP | hsa | [88] |
| 6GEJ | EM (3.6) | Nucleosome complex RUVBL1: V21-L463 (Sequence: M1-L463)/RUVBL2: L14-K460 (Sequence: M1-E471) | ADP | sce | [89] |
| 6GEN | EM (3.6) | Nucleosome complex RUVBL1: V21-L463 (Sequence: M1-L463)/RUVBL2: L14-K460 (Sequence: M1-E471) | ADP | sce | [89] |
| 6H7X | X-ray (2.9) | RUVBL2: S43-I147, K160-K184, Q188-I202, P228-S252, G256-K456 (Sequence: M1-S463) | - | hsa | [90] |
| 6HTS | EM (4.8) | INO80 Complex RUVBL1: T12-Y454 (Sequence: M1-K456)/RUVBL2: D15-P150, S156-N453 (Sequence: M1-S463) | ADP | hsa | [91] |
| 6IGM | EM (4.0) | SRCAP Complex RUVBL1: Q13-E142, I154-P250, T269-I446 (Sequence: M1-K456)/RUVBL2: V16-P150, K157-T183, D191-I202, Q226-A449 (Sequence: M1-S463) | - | hsa | [92] |
| 6K0R* | X-ray (2.5) | RUVBL1 ΔDII: E5-I124, I235-R249, T269-Q451 (Sequence: GPS+K2-E126+GPPG+ I234-K456)/RUVBL2 ΔDII: H25-I131, H240-N251, E267-F450 (Sequence: GGS+M1-E133+GPPG+V238-S463) | ADP, 3AT, CUU, CU0 | hsa | [93] |
| 6QI8 | EM (3.8) | RUVBL1: K2-I120, D237-R249, T272-K456 (Sequence: M1-K456)/RUVBL2: T8-I131, S243-N453 (Sequence: M1-S463) | ADP | hsa | [94] |
| 6QI9 | EM (4.6) | RUVBL1: K2-I120, D237-R249, T272-K456 (Sequence: M1-K456)/RUVBL2: T8-I131, S243-N453 (Sequence: M1-S463) | ADP# | hsa | [94] |
Hsa, cthr, and sce stand for Homo sapiens, Chaetomium thermophilum, and Saccharomyces cerevisiae, respectively.
*
The complex was assembled as Dodecamer
#
The hexameric RUVBL1/2 complex only binds with 5 ADP molecules.
NA: not applicable.
PDB accessed on May 13th, 2020