Complementary patterns of Calca (red) and Pdyn (blue) efferent projection patterns in the preoptic area and BST in Calca-Cre case 1803 and Pdyn-IRES-Cre case 01812 (Huang et al., 2020). (a,d) Calca projections target BSTov and BSTfu. (b,e) Pdyn projections minimally target the BST, and instead heavily target the median preoptic (MnPO), ventromedial preoptic (VMPO), ventrolateral preoptic (VLPO), and parastrial (PS) nuclei, as well as a dorsoventral segment of the preoptic area between the VLPO and PS. (c,f) Combined labeling.