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. Author manuscript; available in PMC: 2022 Aug 1.
Published in final edited form as: J Comp Neurol. 2021 Mar 25;529(11):2911–2957. doi: 10.1002/cne.25136

Figure 4.

Figure 4.

Neurons in the motor trigeminal nucleus (V) and locus coeruleus (LC) express Calca mRNA, produce CGRP, and exhibit Cre-conditional gene expression in Calca-Cre mice. (a,d) In situ hybridization for Calca mRNA (red) in V (a) and in LC (d). (b,e) Immunofluorescence labeling for CGRP (magenta) in V (b) and in LC (e). (c,f) Nickel-DAB (NiDAB) immunohistochemical labeling for mCherry reveals Calca-Cre-conditional expression of synaptophysin(Syp)-mCherry in V (c) and in LC (f) from case 1807, described below. All scale bars are 100 μm.