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. Author manuscript; available in PMC: 2022 Aug 1.
Published in final edited form as: J Comp Neurol. 2021 Mar 25;529(11):2911–2957. doi: 10.1002/cne.25136

Figure 8.

Figure 8.

Strong expression of mRNA for the mu opioid receptor (Oprm1) co-localizes with Calca mRNA in PBeL. (a–d) In situ hybridization for Oprm1 mRNA (ice blue) co-localizes with Calca mRNA (red) in the outer portion of PBeL. Some neurons without Calca also express Oprm1 in the inner portion of PBeL. (e–h) In the LC, Calca mRNA co-localizes with lighter, per-cell Oprm1 expression. (i–l) Motor neurons in V express little to no Oprm1. Scale bars are: 100 μm in (a; also applies to c–d), 100 μm in panel (e; also applies to f–h), and 50 μm in panel (i; also applies to j–l).