Table 2.
Antisera used in this study
| Antigen | Immunogen description | Source, Host Species, RRID | Concentration |
|---|---|---|---|
| DsRed | DsRed-Express, a variant of Discosoma sp. red fluorescent protein | Clontech, rabbit polyclonal, cat. # 632496, lot# 1509043, RRID: AB_1001313483. Immunoreactivity is absent in brain tissue from mice lacking a dsRed-derived fluorescent protein. | 1:2,000 |
| mCherry | Full length mCherry fluorescent protein | Life Sciences, rat monoclonal, cat.# M11217, lot# R1240561, RRID AB_2536611. Immunoreactivity is absent in brain tissue from mice lacking a dsRed-derived fluorescent protein. | 1:2,000 |
| Forkhead box protein 2 (FoxP2) | Recombinant human FOXP2 isoform 1 Ala640-Glu715 | R&D Systems, sheep polyclonal, cat.# AF5647, RRID: AB_2107133. | 1:10,000 |
| ChAT | Human placental choline acetyltransferase | Millipore, goat polyclonal, cat.# AB144P, lot: JC1618187, RRID:AB_2079751. | 1:1,000 |
| CGRP | Synthetic peptide corresponding to Rat CGRP aa 23-37 (C terminal). Conjugated to gamma globulin. Sequence: VKDNFVPTNVGSEAF |
Abcam, goat polyclonal, cat.# ab36001, lot# GR3186077-4, RRID: AB_725807. Neuronal immunoreactivity is absent in homozygous Calca-Cre mice, in which a Cre:GFP knockin to exon 2 of the Calca locus disrupts CGRP production (see Supplemental Figure 1). | 1:8,000 |
| CGRP | Synthetic CGRP peptide | Peninsula, rabbit polyclonal, cat.# T-4032, lot# A17601, RRID: AB_518147. Neuronal immunoreactivity is absent in homozygous Calca-Cre mice, in which a Cre:GFP knockin to exon 2 of the Calca locus disrupts CGRP production (see Supplemental Figure 2). | 1:16,000 |
| Cre | Cre recombinase from Escherichia phage P1 | Novagen/Millipore, rabbit polyclonal, cat.# 69050-3, lot# 2811981, RRID: AB_10806983. Immunoreactivity is absent in brain tissue from mice without Cre recombinase. | 1:10,000 |
| GFP | GFP isolated directly from the jellyfish Aequorea victoria. | Invitrogen, chicken polyclonal, cat.#10262, lot: 1829565, RRID: AB_2534023. | 1:3,000 |
| LIM homeobox transcription factor 1 Beta (Lmx1b) | Full-length LIM homeobox transcription factor 1 beta protein from mouse | C. Birchmeier, Max Delbruck Center for Molecular Medicine; Berlin; Germany. RRID: AB_2314752. | 1:8,000 |
| Tyrosine hydroxylase (TH) | Purified, SDS-denatured rat pheochromocytoma TH | Millipore, mouse monoclonal, cat. # MAB318, lot: NG1802536, RRID: AB_2201528. | 1:10,000 |