Figure 3.

Effect of Anti–IL-6-Ab and sgp130Fc on Soluble Inflammatory Mediators and Leukocytes After MI With Reperfusion

Rats were subjected to surgical myocardial infarction with 50 min of ischemia before reperfusion. One minute before reperfusion, vehicle (phosphate-buffered saline solution [PBS]), anti–IL-6-Ab (0.1 μg/mg, second dose intraperitoneally 3 days after MI), or sgp130Fc (0.5 μg/mg) was administered intravenously in 1 ml PBS (n = 5–9/group). (A to D) Cytokines in plasma and supernate of myocardial digests were measured by means of enzyme-linked immunosorbent assay and enzyme immunoassay. (E to K) Cell concentrations and CD11b expression were measured by means of flow cytometry. Data presented as mean ± SEM. Statistical significance was tested by means of analysis of variance (ANOVA) with multiple comparisons (comparing drug groups with vehicle; black asterisks and daggers) or unpaired Student’s t-tests (comparing sham with vehicle controls; gray asterisks and daggers). Where ANOVA was significant but differences between vehicle and the drug groups were not, a post hoc unpaired Student’s t-test was performed between the two drug groups (red asterisk). Multiplicity-adjusted (asterisks) and ANOVA (daggers) p < 0.05 was considered to be significant. ∗†p<0.05; ∗∗††p<0.01; ∗∗∗†††p<0.001. Ab = antibody; MHC = major histocompatibility complex; Mo = monocytes; other abbreviations as in Figures 1 and 2.