Table 2.
Gradient elution conditions for high-performance liquid chromatography.
| Time (Min) | 0.5% acetic acid water (%) | Acetonitrile (%) |
|---|---|---|
| −10 | 85 | 15 |
| 0 | 85 | 15 |
| 28 | 65 | 35 |
| 39 | 58 | 42 |
| 40 | 85 | 15 |
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