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. 2021 May 17;9:670145. doi: 10.3389/fcell.2021.670145

FIGURE 5.

FIGURE 5

B7H3 downregulation is associated with reduced expression of VEGFA in IDH-mutated gliomas. (A,B) VEGFA is reduced accompanied by decreased B7H3 expression in IDH1-R132H U87 and U251 cells. Stably expressed IDH1-WT, IDH1-R132H, and IDH1-R132H/T77A U87 and U251 cells were collected and immunoblotting was performed with the indicated antibodies. All experiments were performed in triplicate and representative results were shown. (C) Absolute 2-HG quantification in IDH1-mutated gliomas. N = 9 in 2-HGhigh and n = 8 in 2-HGlow IDH1-mutated gliomas, respectively. ***denotes the P < 0.001 for the indicated comparison. (D,E) B7H3 is positively associated with VEGFA in gliomas. IDH1-WT (n = 4), 2-HGlow IDH1-mutated gliomas (n = 8), and 2-HGhigh IDH1-mutated gliomas (n = 9) were used for IHC staining with the indicated antibodies (D), scale bar = 50 μM. Quantifications of B7H3 and VEGFA staining scores were shown in (E). *denotes the P < 0.05, **denotes the P < 0.01, and ***denotes the P < 0.001 for the indicated comparisons.