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. 2021 May 17;12:667146. doi: 10.3389/fmicb.2021.667146

FIGURE 3.

FIGURE 3

Silencing of MX1 and IFIT1 enhances peak ZIKV replication in Sertoli cells. SC were transfected with siRNA for MX1 (siMX1), IFIT1 (siIFIT1), or negative control (siControl) 24 h prior to ZIKV infection (MOI 1). (A) The silencing of MX1 (siMX1) and (B) IFIT1 (siIFIT1) was evaluated at 48 and 96 h post-infection by measuring MX1 and IFIT1 expression, respectively, in mock SC, determined by RT-qPCR and reported as fold-change compared to siControl. (C,D) ZIKV infectious progeny measured in infected SC transfected with siControl and siMX1 or with siControl and siIFIT1 by plaque assay. (E,F) ZIKV genome copies in infected siControl were compared to (E) infected siMX1 and (F) infected siIFIT1 by RT-qPCR. The housekeeping gene GAPDH was used to normalize fold-change for all gene expression assays. Significance (n = at least 3 for each condition at each time point) determined by Student’s t-test for all assays, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.