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. 2021 May 17;12:667146. doi: 10.3389/fmicb.2021.667146

FIGURE 4.

FIGURE 4

IFN response is less robust in Sertoli cells as compared to brain microvascular endothelial cells and A549 lung epithelial cells. (A) Human SC, BMVEC, and A549 cells were treated with 30 pg/mL (6 IU/mL) of recombinant human IFN-β (rhIFN-β; R&D systems), RNA was extracted from cell lysates 24 h later, and gene expression of ISGs (MX1, IFIT1, and ISG15) was measured by RT-qPCR (n = 3 for each infected and mock samples). (B,C) SC and A549 cells were infected with ZIKV at MOI 1. (B) ZIKV progeny was measured in the culture supernatant by plaque assay at 24 and 48 h post-infection. (C) Expression of mRNA transcripts for type I IFN (IFNB1), type III IFN (IFNL3), MX1, IFIT1, and ISG15 in ZIKV-infected SC and A549 cells compared to respective mock samples was measured by RT-qPCR at 48 h post-infection (n = 3 for each infected and mock samples). The housekeeping gene GAPDH was used for normalization for all gene expression analyses. Significance determined by Student’s t-test for all assays, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.