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. 2021 May 31;7:127. doi: 10.1038/s41420-021-00510-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Analysis of cell proliferation/viability by MTT assay of M14 cells treated with 61 essential oils (EOs, EO01-EO61, 50 μg/ml, 72 h). p-values were calculated between control (Ctrl) and EO-treated cells. *p < 0.05; **p < 0.01; ^#p < 0.001 after applying Student’s t test. Dotted columns represent the six EOs further investigated in this study. b MTT assay of M14 cells treated with the indicated EOs (10–50 μg/ml, 24–72 h). a, b Results are reported as “cell proliferation-viability of treated cells/cell proliferation-viability of control cells × 100” and represent the average±standard deviation of at least three independent experiments. c Quantification of 50% inhibition of cell proliferation/viability (IC₅₀) of the indicated EOs calculated for M14 cells treated as reported in b. The median of IC₅₀ is shown. ***p < 0.001; ****p < 0.0001 after applying one-way ANOVA test.