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. 2021 May 31;7:127. doi: 10.1038/s41420-021-00510-3

Fig. 5. Terpinen-4-ol is responsible for EO05 antitumor activity in M14 cells.

Fig. 5

a MTT assay of M14 cells treated for 72 h with eucalyptol (7 μg/ml), γ-terpinene (6 μg/ml), α-terpineol (4 μg/ml), terpinen-4-ol (18.5 μg/ml) or EO05 (50 μg/ml). b MTT assay of M14 cells treated with the indicated concentrations of EO05 or of terpinen-4-ol. c, d MTT assay (left) and relative isobologram (right) of M14 cells treated with c dabrafenib (DAB), d trametinib (TRAM), or terpinen-4-ol alone or in combination (24 h terpinen-4-ol followed by 48 h DAB or TRAM). a–d The results are reported as “cell proliferation-viability of treated cells/cell proliferation-viability of control cells × 100”. The results represent the average±standard deviation of at least three independent experiments. p-values were calculated between control (Ctrl) and treated cells or cells treated in combination and cells treated with single drugs. *p < 0.05; **p < 0.01, after applying Student’s t test.