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. 2021 May 17;12:580761. doi: 10.3389/fgene.2021.580761

TABLE 2.

Summary of previous genetic studies of hemifacial microsomia.

Study Mutated gene(s) symbol Abnormal chromosomal region(s)/chromosome abnormality Mutation type Utilized analysis method
Bragagnolo et al., 2018 HMX-1, BAPX1, EYA1, PUF60, NRBP2, SCRIB, GATA3, ATR-16, NF1, PRKX, RAS2 4p16.1, 4p16.3p15.33, Xp22.33–p22.31, 22q11.21, 8q13.3, 8q24.3, 10q26.2q26.3, 10p13p14, 16p13.3, 16p13.11p12.3, 17q11.2, Xp22.33 CNV (dup/del) Karotyping with G-banding, CMA
Spineli-Silva et al., 2018 YPEL1, MAPK1, ERK2, GSC2 22q11.2 CNV (del) MLPA, CMA
Berenguer et al., 2017 MYT1 20q13.33 SNV (c.323C>T, p.Ser108Leu) SNP array
Lopez et al., 2016 MYT1 20q13.33 SNV (c.314C > T, p.Ser105Leu) WES, transient knockdown in zebrafish
Colovati et al., 2015 ZNF74, KLHL22, MED15, SNAP29, LZTR1 22q11.21 CNV (del) Genomic array analysis, MLPA
Zhang et al., 2016 ROBO1, GATA3, EPAS1, PARD3B, GBX2, SHROOM3, FRMD4A, FGF3, KLF12, EDNRB, NID2, SEMA7A, PLCD3 3p12.3, 10p14, 2p21, 2q33.3, 2q37.2, 4q21.1, 10p13, 11q13.3, 13q22.1, 13q22.3, 14q22.1, 15q24.1, 17q21.31 UK SNP, GWAS, GRAIL, DEPICT, qPCR, WGS
Beleza-Meireles et al., 2015 Not determined 22q11 CNV (dup/del) aCGH
Guida et al., 2015 ATP13A3, XXYLT1 3q29 CNV (dup) SNP array
Zielinski et al., 2014 OTX2 14q22.3 CNV (dup) WES, SNP array
Torti et al., 2013 Not determined 22q11.2 CNV (dup/del) aCGH, FISH
Ballesta-Martinez et al., 2013 OTX2 14q23.1 CNV (dup) Linkage analysis in families with autosomal dominant inheritance, aCGH
Quintero-Rivera and Martinez-Agosto, 2013 Not determined 22q11.1–q11.21 CNV (tetrasomy) FISH, aCGH
Su et al., 2012 TCOF1 5q32–q33.1 UK PCR, direct sequencing
Rooryck et al., 2010 IQSEC3, SLC6A12, SLC6A13, JARID1A, CCDC77, B4GALNT3, NINJ2, WNK1, HSN2, RAD52, ERC1, FBXL14, WNT5B, ADIPOR2, CACNA2D4, LRTM2, DCP1B, CACNA1C, SPRY2 12p13.33, 47, XXX, Yp–q11.221. Yq11.222–q12, t(9;18) (p23;q12,2), 13q13.1 CNV (del/dup/trisomy/translocation) aCGH, QMF-PCR
Northup et al., 2010 Not determined inv(14) (p11.2q22.3) CNV (inversion) FISH
Huang et al., 2010 BIR1C, OCLN 5q13.2 CNV (del) Illumina HumanCNV370 Genotyping BeadChip, qPCR
Rooryck et al., 2009 WNT5B, CACNA1C del(12) (pter/p13.33) CNV (del) aCGH, QMF-PCR
Alasti and Van Camp, 2009 BAPX1, GSC, Hfm 4p15.33, 14q32.13, 14q32 UK Review
Ala-Mello et al., 2008 Not determined 5p15.3–pter, 21q22.3–qter, 21q22.11q22.12. CNV (del/dup) FISH, aCGH
Ou et al., 2008 SIX1, SIX6, OTX2 14q22.3–q23.3; 13q21.31-q21.32 CNV (del/dup) Karotyping, FISH, aCGH
Kosaki et al., 2007 SALL1 16q12.1 SNV (c.1256T > A, p.L419X) PCR, direct sequencing
Zhu et al., 2007 ZIC3 Xq26.3 UK Analysis of Zic3 null mice
Terhal et al., 2006 SALL4 (exon 3) 20q13.2 UK PCR, direct sequencing

aCGH, array-based comparative genomic hybridization; CMA, chromosomal microarray analysis; CNV, copy number variation; del, deletion; DEPICT, data-driven expression-prioritized integration for complex traits; dup, duplication; FISH, fluorescence in-situ hybridization; GRAIL, gene relationships across implicated loci; GWAS, genome-wide association study; MLPA, multiplex ligation-dependent probe amplification; PCR, polymerase chain reaction; QMF-PCR, quantitative multiplex fluorescence-PCR; qPCR, quantitative PCR; SNP, single nucleotide polymorphism; SNV, single nucleotide variant; UK, unknown; WES, whole-exome sequencing; WGS, whole-genome sequencing. XXX means XXX syndrome, also known as Triple X syndrome, and trisomy X, which is a rare and genetic disease. It is characterized by the presence of an extra X chromosome in each cell of a female.