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. 2021 May 17;12:685724. doi: 10.3389/fimmu.2021.685724

Figure 2.

Figure 2

NF-κB pathway activation and NLRP3 inflammasomes formation were promoted in ovarian granulosa cells from PCOS patients. (A) Identification of GCs in primary cells extracted from ovaries of PCOS patients and controls by immunofluorescence assays. (FSHR, red; DAPI, blue; scale bar, 20 μm). (B) The mRNA levels of TLR4 between PCOS patients and controls in GCs were measured by RT-qPCR assays (P = 0.0184). (C) The mRNA levels of p65 between PCOS patients and controls in GCs were measured by RT-qPCR assays (P = 0.0292). (D) The phosphorylation levels of p65 were measured by western blotting assays. (E) The mRNA levels of NLRP3 in GCs in PCOS patients and controls were measured by RT-qPCR assays (P = 0.0096). (F) NLRP3 protein levels in GCs in PCOS patients and controls by western blotting assays. (G) The mRNA levels of IL-1β from PCOS patients and controls by RT-qPCR assays (P = 0.0005). (H) The levels of NLRP3 inflammasome-related proteins (ASC, pro-caspase-1, and caspase-1) were measured by western blotting assays. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001. *P < 0.05 was considered statistically significant.