Figure 4.
Analysis of laser wounding experiments
(A) Laser wounding in monolayers of MDCK cells expressing E-cadherin-RFP for uninfected (top) or L. m.-infected wells (bottom) 24 hpi. Columns show: the brightfield image superimposed with L. m. fluorescence (green) prior to wounding (t=0 min); the corresponding E-cadherin localization prior to wounding (t=0 min); E-cadherin localization immediately after 1 min of laser exposure (t=1 min); the cell displacement vectors u (50-fold larger) calculated by performing PIV; the average uy at t=1 min (positive/negative values point towards/away from wound); and the average uy over the time period 30–40 min.
(B) Kymographs of the average with respect to the wound displacement vectors, uy as a function of time, t (min, x-axis) and vertical position, y (μm, y-axis) for the same examples as shown in panel A (see single and double crosses).
(C) Barplots of the mean uy along a distance of 100 μm away from the wound and calculated immediately after wounding, for cells originating from uninfected or from infected wells (N= 3 experiments, mean+/-SD, unpaired Student’s T-Test: ∗ p<0.01).
Parts of the figure reprinted with permission from (Bastounis et al., 2021).