REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, peptides, and recombinant proteins
40% Acrylamide solution	Sigma	Cat#A4058
2% Bis-acrylamide solution	Thermo Fisher	Cat#BP1404-250
Sulfo-SANPAH	Thermo Fisher	Cat#22589
FluoSpheres™ Carboxylate-Modified Microspheres, 0.2 μm, yellow-green fluorescent (505/515), 2% solids	Invitrogen	Cat#F8811
Rat tail collagen type I	Sigma	Cat#C3867
HEPES, free acid	J.T. Baker	Cat#4018-04
(3-Aminopropyl)triethoxysilane	Sigma	Cat#3648
Ammonium persulfate	Fisher	Cat#BP17925
TEMED	Sigma	Cat#T9281
25% Glutaraldehyde	Sigma	Cat#G6257
Leibovitz's L-15 Medium, no phenol red	Thermo Fisher	Cat#21083027
High-glucose DMEM medium	Thermo Fisher	Cat#11965092
Foundation Fetal Bovine Serum, lot: A37C48A	Gemini Bio-Prod	Cat#900108
Brain heart infusion (BHI)	BD	Cat#211059
Phosphate buffered saline (PBS), no calcium, no magnesium	Fisher	Cat#SH30028FS
Gentamicin sulfate	MP Biomedicals	Cat#194530
Chloramphenicol	Sigma	Cat#C0378-5G
Streptomycin sulfate	Thermo Fisher	Cat#3810-74-0
DifcoTM agar, granulated	BD	Cat#214530
0.25% Trypsin-EDTA, phenol red	Thermo Fisher	Cat#25200056
Hoechst 33342, trihydrochloride, trihydrate - 10 mg/μL	Invitrogen	Cat#H3570
Formaldehyde 16% EM grade	Electron Microscopy Sciences	Cat#15710-S
Experimental models: Cell lines
MDCK II	Bakardjiev Lab (Faralla et al., 2018)	N/A
MDCK II E-cadherin-RFP	Nelson Lab (Perez et al., 2008)	N/A
Experimental models: Organisms/strains
L. monocytogenes: L.m.-ActAp::mTagRFP	Theriot Lab(Ortega et al., 2017)	N/A
L. monocytogenes: L.m.-GFP	Theriot Lab (Ortega et al., 2017)	N/A
Software and algorithms
ImageJ	(Schneider et al., 2012)	https://imagej.nih.gov/ij/
Micro-Manager	N/A	https://www.micro-manager.org/
MATLAB	MathWorks	http://www.mathworks.com/products/matlab/?requestedDomain=www.mathworks.com
Mound volume measurement code	This paper	https://gitlab.com/theriot_lab/theriot-toolbox/infection-mound-volume
Laser wounding recoil displacement code	This paper	https://gitlab.com/theriot_lab/infection_mound_laser_wounding
GraphPad Prism v6	GraphPad	http://www.graphpad.com/scientific-software/prism/
Imaris	Bitplane	https://imaris.oxinst.com/
Other
24-Well plates with glass bottom	MatTek	Cat#P24G-1.5-13-F
T-75 Flasks	Falcon	Cat#353118
Plastic cuvettes	Sarstedt	Cat#67.746
50 mL Conical tubes	Falcon	Cat#352070
15 mL Conical tubes	Falcon	Cat#352196
Disposable serological pipettes (1 mL, 2 mL, 5 mL, 10 mL)	Falcon	various
Bacterial cell spreader	Sigma	Cat#Z376779
Pipette tips 1-200 μL	Denville	Cat#P1122
Pipette tips 101–1000 μL	Denville	Cat#P1126
Powder-free examination gloves	Microflex	Cat#XC-310
0.2 μm Vacuum bottle top filters	Thermo Fisher	Cat#566-0020
20 mL Syringes	BD	Cat#302830
0.2 μm Syringe filters	Thermo Fisher	Cat#723-2520
10 cm Culture plates	Falcon	Cat#351029
Biosafety cabinet	Baker	Cat#SG504
Hemocytometer	Sigma	Cat#Z359629
Bacterial incubator	Thermo Scientific	Cat#IGS180
CO2 culture incubator	NuAire	Cat#NU-8700
Vacuum chamber/degasser	Bel-Art	Cat#42025
Cage incubator (for the microscope)	Haison	Custom
Pipettor	Drummond	Cat#4-000-110
Pipet tips (10 μL, 200 μL, 1000 μL)	Gilson	Cat#F144802, F123601, F123602
pH meter	Mettler Toledo	Cat#30019028
1 L Flask	Fisherbrand	Cat#FB5011000
Autoclave	Amsco	Cat#3021
Stir magnet plate	Bellco	Cat#7760-06000
Magnet stirring bars	Bellco	Cat#1975-00100
Spectrophotometer	Beckman	Cat#DU 640
Firefly system	Rapp OptoElectronic	Cat#UGA-42 Firefly