Table 1.
Primers and Probes Used for the Detection of HF183 and PhiX174
| Assay | Primers/probe sequences (5′ → 3′) | Amplicon length (bp) | References |
|---|---|---|---|
| HF183/BacR287 | F primer ATCATGAGTTCACATGTCCG | 191 | Green et al. (2014) |
| R primer CTTCCTCTCAGAACCCCTATCC | |||
| Probea/6-FAM/ CTA+ATGGAAC+G+CA+T+CCC/IABkFQ/ | |||
| PhiX174 | F primer (ΦX174): CGCCATTAATAATGTTTTCCGTAA | 73 | Myers et al. (2009) |
| R primer (ΦX174): CATCCCGTCAACATTCAAACG | |||
| Probe (ΦX174):/6-FAM/ CGCCTTCCATGATGAGA/MGB/ |
+, Indicates locations of extra methylene bridges.
a
LNA probe (Integrated DNA Technologies).
6-FAM, 6-carboxyfluorescein (on 5′ end); IABkFQ, Iowa Black® FQ quencher (on 3′ end); LNA, locked nucleic acid; MGB, minor groove binder.