Supplemental Figure S1.

Subsample interpolation and ABC calculation. Both panels show a 16-cell subsampling from a larger population of measurements made with slope = 1 and SD = 0.5. The trajectory of these 16 data points is interpolated with cellAlign and shown in a black line. In the left panel, the subsample’s interpolated trajectory is compared with the true trajectory derived from the original population (blue line). The area between these two lines is calculated as the ABC_true (shaded in gray). In the right panel, a null model of no change is evaluated. The subsample’s trajectory is compared with a flat line equivalent to the average intensity value; this metric is called ABC_null.