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. 2021 Apr 30;296:100736. doi: 10.1016/j.jbc.2021.100736

Figure 2.

Figure 2

Kinetics of sulfide disappearance under different experimental conditions.A, kinetics of Na2S (300 μM) disappearance from 6-cm culture plates containing PBS, or complete RPMI 1640 medium with or without HT29scr cells. B and C, changes in the concentration of cysteine (B) and sulfane sulfur (C) in the same experiments as in A as well as in the presence of cells but absence of Na2S. D, kinetics of sulfide (100 μM) disappearance from modified PBS (DPBS) in the presence or absence of a 5% suspension (w/v) of HT29scr cells or in the presence of HT29 cells with SQOR or ETHE1 knocked down. E, accumulation of thiosulfate in the experiments shown in (D). Data represent the mean ± SD of 3 to 5 experiments. F, Western blot (left) showing knockdown of ETHE1 in HT29 cells using two shRNA sequences and Ponceau S staining (right) for equal loading.