Figure 3.
Sulfide enhances aerobic glycolysis. Kinetics of glucose consumption (A) and lactate accumulation (B) in 5% suspension (w/v) of HT29scr cells in modified PBS (DPBS) in the absence (gray) or presence (blue) of 100 M Na2S. The data are representative of five independent experiments, each with technical duplicates. C and D, Na2S (100 μM) increases glucose consumption (C) and lactate production (D) rates in all but HCEC cell lines in 5% suspensions (w/v) in DPBS. E, at a higher concentration of Na2S (200 μM), increased glucose consumption and lactate production are also observed in HCEC cells. F, the lactate:glucose ratio obtained in the experiments is presented in C and D. The error bars represent the SD on the mean of 3 to 9 experiments. ∗Indicates statistically significant difference from control cells (p < 0.05).