Figure 6.

Involvement of the mitochondrial NADH pool in signaling sulfide effects on glycolysis.A, the reaction catalyzed by LbNOX. B, the electron transport chain where CI–CIV denote complexes I–IV and QH₂ and Q denote reduced and oxidized CoQ. C–E, the effect of Na₂S (100 μM) on the rates of glucose consumption (C) and lactate production (D), and the lactate:glucose ratio (E) in HT29 cells expressing empty vector (EV) or LbNOX (cytoplasmic or mitochondrial) and in wildtype (143B^wt) or mutant (143B^Cytb) 143B cells. ∗Indicates statistically significant difference from the corresponding (-Na₂S) controls, p < 0.05. ^#Indicates statistically significant difference from empty vector control, p < 0.03. The error bars represent the SD on the mean of 3 to 8 independent experiments.