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Effect of different light regimes on the mRNA expression levels of CaBP-D28k, PMCA1b and OPN and the protein expression level of CaBP-D28k and PMCA1b in the eggshell gland. Relative mRNA expression level of CaBP-D28k (A), PMCA1b (B) and OPN (C) were evaluated by RT-PCR. Western blot analysis for detection of CaBP-D28k and PMCA1b (D, E, F). Data represent the mean ± SD (n = 8); ∗P < 0.05.
