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. 2021 May 18;64(10):6706–6719. doi: 10.1021/acs.jmedchem.0c02177

Figure 1.

Figure 1

Diagram of constructs used to identify May1 architecture and optimize expression strategy. Residue numbers indicate construct boundaries. The first 17 N-terminal amino acids, forming a putative native secretion signal, were replaced with a secretion signal compatible with the S2 expression system (not shown for clarity). Beveled rectangles denote positions of affinity tags used for purification.