Skip to main content
. 2021 May 18;64(10):6706–6719. doi: 10.1021/acs.jmedchem.0c02177

Figure 2.

Figure 2

Analysis of May1(17-434)-Avi expression and purification. (a) Silver-stained SDS-PAGE of samples collected throughout the purification process. MED, crude S2 media; PER, permeate from media concentration; LOAD, concentrated filtrate; FT, flow-through; W1-3, wash fractions; E1-4, elution fractions; POOL, pooled concentrated elution fractions; MTX, matrix binding control. (b) Western blot analysis with the same layout as in a visualized using Streptavidin-800CW (LI-COR, 1:15,000). (c) Silver-stained SDS-PAGE of concentrated pooled fractions after size exclusion chromatography.