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. 2021 May 30;19:229. doi: 10.1186/s12967-021-02872-9

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — UCA1 acts as a ceRNA of miR-122-5p to affect SOX2 expression. a Prediction of subcellular localization of UCA1 by online analysis website. b FISH assay verified the subcellular localization of UCA1. c Prediction of binding sites between UCA1 and miR-122-5p in RNA22 website. d Dual luciferase reporter gene assay detected the binding site of UCA1 to miR-122-5p. e RNA pull-down assay verified the enrichment of UCA1 to miR-122-5p. f Prediction of the target relationship between miR-122-5p and SOX2 by online website. g Dual luciferase reporter gene assay detected the target relationship between miR-122-5p and SOX2. * P < 0.05 vs. the mimic-NC group, # P < 0.05 vs. the Bio-miR-122-5p-Wt group. Measurement data were depicted as mean ± standard deviation, comparisons between two groups were assessed by independent sample t test, and comparisons among multiple groups were assessed by one-way analysis of variance followed with LSD-t, the experiment was repeated three times