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. 2021 Mar 26;49(3):0300060521996564. doi: 10.1177/0300060521996564

Figure 3.

Figure 3.

circPPP1CC targets miR-107 and miR-103a-3p and inhibits their expression. (a) Silencing of circPPP1CC with siRNA confirmed by RT-PCR. (b) Overexpression of miR-103a-3p and miR-107 with mimics detected using RT-PCR. (c) Predicted binding sites between circPPP1CC and miR-107 or miR-103a-3p. (d) Luciferase activity in NC, miR-107, and miR-103a-3p mimics groups. (e) Relative expression of miR-107 and miR-103a-3p in HUVECs, detected by RT-PCR following cell stimulation with Pg-LPS and transfection with siRNA targeting circPPP1CC. *P < 0.05, **P < 0.01, ***P < 0.001; error bars represent standard deviation.

circPPP1CC, circular RNA serine/threonine-protein phosphatase PP1-gamma catalytic subunit; siRNA, short interfering RNA; NC, negative control; RT-PCR, reverse transcription PCR; HUVECs, human umbilical vein endothelial cells; Pg-LPS, Porphyromonas gingivalis-lipopolysaccharide.