Fig. 4.

ACSL4 inhibited cell survival and tumor invasion/migration and promoted ferroptosis in lung adenocarcinoma. a The expression of ACSL4 in 4 different lung adenocarcinoma cell lines after ACSL4 knockdown (shACSL4) or overexpression (oeACSL4). Representative Western blot results were shown and ACTIN was used as the loading control. b Cell viability was analyzed by MTT in lung adenocarcinoma cells after ACSL4 knockdown or overexpression (n = 3). c Cell migration and invasion ability was analyzed by the transwell assay in lung adenocarcinoma cells after ACSL4 knockdown or overexpression. The quantification of the transwell assay for each cell line was shown on the right (n = 3). d Cell migration was analyzed by wound healing assay in lung adenocarcinoma cells after ACSL4 knockdown or overexpression (n = 3; *p < 0.05, **p < 0.01, ***p < 0.001). The multiple comparisons of student’s t-test (two-tailed) were corrected using the Bonferroni method. ns, not significant. e Liperfluo-based lipid peroxidation assay was used to monitor the ferroptosis levels in lung adenocarcinoma cells after ACSL4 knockdown, ACSL4 overexpression, and/or erastin treatment