Fig. 8.

Effect of SNV-mediated loop change and G-tract disruption on IRF8 gene expression. (A) Formation of G4s near the TSS of the IRF8 gene detected by G4-ChIP in living human HEK293T cells. The red arrowhead indicates the PQS bearing the SNVs. (B) Formation of G4s in the REF and VAR duplex DNAs detected by native gel electrophoreses in the PQS indicated by an arrowhead in A. The DNA was heated (H) to generate G4 or not heated (N) to remain fully annealed. (C) Same as B, except that G4 formation was detected by DMS footprinting. (D) Digitization of C. (E) RNA and (F) protein expression of the luciferase reporter downstream of the IRF8 promoter in a pGL3-basic plasmid transfected into HEK293T cells.