Fig. 4.

TIGIT signaling inhibits the AKT-mTORC1 pathway to down-regulate Foxp3 expression. (A–D) WT iTreg cells were stimulated by using anti-CD3 mAb or isotype control antibody plus either anti-TIGIT mAb or isotype control antibody. On day 3 after stimulation, (A and B) Foxp3 expression and phosphorylation of (C) S6 and (D) AKT were analyzed by qRT-PCR and flow cytometry, respectively. (Left) Representative histograms and (Right) statistical results are shown. The data shown are combined from two independent experiments. Data in graphs are given as means ± SD; (A–D) one-way ANOVA, ***P < 0.001.