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. 2021 May 31;19:162. doi: 10.1186/s12951-021-00909-1

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — CuONPs instillation caused acute lung injury dose-dependently in mice. A Representative H&E staining images of lung tissues of mice 3 day after intratracheally administration of vehicle, 30, 50 or 100 μg CuONPs, respectively. Scale bar, 200 μm in the upper panels and 50 μm in the bottom panels. B The lung injury scores of H&E staining were calculated. C The cell counts in BALFs collected from indicated groups were determined by automated cell counter. D The protein concentrations of BALF collected from indicated groups were determined by BCA analysis. E The LDH activities of BALFs collected from indicated groups were determined. *indicates p < 0.05 compared with vehicle group