Fig. 4.

CuONPs instillation caused oxidative stress in lung tissue of mice. Mice were intratracheally instilled with vehicle, 1.5, 2.5 or 5 mg/kg CuONPs, respectively for 3 days. A The mRNA expression levels of Nfe2l2, Hspa5, Hmox1 and Gclm in mice lung tissues in the indicated groups were determined by QPCR analysis. B The protein expression levels of NRF2, HMOX1, GCLM and BIP in mice lung tissues in the indicated groups were determined by western blot analysis. β-actin was served as internal control. C Quantification of the relative protein expression levels shown in B *indicates p < 0.05 compared with vehicle group