Fig. 7.

Autophagy-related protein changed upon CuONPs instillation in lung tissue of mice. A, B Mice were intratracheally instillation with vehicle, 1.5, 2.5 or 5 mg/kg CuONPs, respectively. After 3 days, the protein expression levels of p-mTOR, mTOR, p-AMPKα and AMPKα in the lung tissues were determined by western blot analysis. β-actin was served as internal control and the indicated protein expression levels were quantified. C, D The protein expression levels of p-mTOR, mTOR, p-AMPKα and AMPKα in the lung tissues were determined by western blot analysis. β-actin was served as internal control and the indicated protein expression levels were quantified. *indicates p < 0.05 compared with vehicle group