Fig. 2.

Characterization of purified recombinant proteins. (A) Silver-stained SDS/PAGE (4 to 20% Tris) of our purified recombinant fusion proteins and Eylea. A total of 200 ng of each protein was subjected to electrophoresis under reducing conditions. Staining was performed by SilverQuest Silver Staining Kit (Invitrogen). (B) Analytical SEC of V23, V233, V1233, and Eylea, 25 μg of each. The y axis represents intensity of absorbance (A₂₈₀) in milli-absorbance units, and the x axis represents elution time in minutes.