Fig. 3.

IC₅₀ values of the inhibitors. Bovine choroidal ECs were maintained as described in Materials and Methods. For assays, cells are plated at low density. Inhibitors were then added at various concentrations as indicated. VEGF₁₆₅ was added at the final concentration of 10 ng/mL. Cell densities were evaluated after 5 d. IC₅₀ values were calculated using GraphPad Prism 5 (GraphPad Software). Data shown are based on two independent experiments obtained with highly purified proteins and are consistent with numerous previous assays.