Fig. 2.

IL-1β, a proinflammatory cytokine, is robustly down-regulated by PDZ1i in tumor cells. (A) DMSO- or PDZ1i-treated (12 h) 4T1-Luc conditioned media were analyzed for expression of various growth factors/cytokines using protein-based cytokine arrays from R&D Biosystems. Data were captured and band intensity was measured using ImageJ software. Relative intensity changes (percentage of DMSO-treated group) are presented for representative cytokines that showed 50% or greater decreases in expression. (B) 4T1-Luc and two different human breast cancer cell lines, MDA-MB-231 and ZR-75-1, were treated for 12 h with PDZ1i and total RNA was prepared. qPCR was performed for selective cytokines (from A) and presented as relative fold-changes vs. the control (DMSO) group. *P < 0.05 is considered as statistically significant.