Fig. 4.
PDZ1i-mediated metastasis suppression is nullified in IL-1β overexpressing 4T1-Luc cells. (A and B) A cohort of animals (n = 20) were inoculated intravenously with either 4T1-Luc control (n = 10) or 4T1-LucIL-1β (n = 10) cells. After 12 h, half of the animals (n = 5) received either DMSO or PDZ1i (30 mg/kg body weight) intraperitoneally during the 2-wk study. Animals were subjected to live imaging on day 7 and day 15. Representative BLI imaging (Upper) and the average ROI (Lower) from each experimental group are presented. (C) Lungs were excised, photographed for gross pathology, and processed for histology (H&E-stained sections) and are presented (Upper and Lower, respectively). Magnification, 20×. (D) Three lungs from each group were processed and analyzed for the presence of G-MDSCs. The average values in different experimental conditions are plotted. *P < 0.05 is considered as statistically significant.