Fig. 6.
PDZ1i-mediated deactivation of STAT3 down-regulates IL-1β transcription. (A and B) Indicated cells were transfected with a control vector or constitutively active STAT3 expression plasmid and 24 h posttransfection, cells were treated with DMSO or PDZ1i. Total cellular RNA and proteins were prepared and analyzed by qPCR and ELISA, respectively. Relative fold-changes of mRNA are presented. The absorbance value represents the amount of protein determined by ELISA. (C) mda-9/Syntenin expression was induced in T47D cells and cells were treated with Stattic, a STAT3 inhibitor. Total RNA and protein lysates were prepared and analyzed for IL-1β at mRNA and protein levels, respectively. (D) IL-1β expression in conditioned media from 4T1-Luc and 4T1-LucSTAT3 cells expressing an active STAT3 expression construct. (E) Indicated cells were treated with DMSO or PDZ1i for 24 h and Western blotting analysis was done with selected antibodies as shown in the figure. *P < 0.05 is considered as statistically significant.