Fig. 7.

Antimetastatic activity of PDZ1i is partially prevented in constitutively active STAT3-overexpressing 4T1-Luc cells (4T1-Luc ^STAT3). (A and B) A cohort of 20 animals were randomly divided into two groups and inoculated with control or STAT3-overexpressing cells. After 12 h, half of these mice received DMSO intraperitoneally and the remaining half received PDZ1i intraperitoneally at 30 mg/kg body weight (three times per week, total six injections). Mice were imaged once a week (on day 7 and day 15) before terminating the study. Representative photomicrographs from different experimental groups are presented (Upper). ROI values were calculated and the average value from five mice are presented for the two time points (Lower). Different letters indicate statistical significance among the corresponding groups. (C) Lungs were excised and representative photographs are presented (Upper). H&E sections covering the lungs in lower magnification are shown (Lower). Magnification, 20×. (D) Accumulation of MDSCs in the lung from different experimental groups were analyzed using FACS and the average values are plotted. Different letter in two variables are statistically significant (P < 0.05).