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. 2021 Feb 12;7:177–196. doi: 10.3114/fuse.2021.07.09

Table 3.

Primer names and sequences used for this study, including the new primers ITS5_hormo, ITS4_hormo and LROR_hormo and the Tulasnella-specific ITS4 primer, ITS4_tul, with melting temperature (Tm) in degrees Celsius. Point mutations are in bold, nucleotide additions are in bold and underlined and nucleotide deletions are shown as a blank space with a bold underline.

Sequence (5’ to 3’) Tm (°C) Reference
V9G TTACGTCCCTGCCCTTTGTA 56 de Hoog & Gerrits van den Ende (1998)
ITS5 GGAAGTAAAAGTCGTAACAAGG 51 White et al. (1991)
ITS5_hormo GGAAGTACAAGTCGTAACAAGG 53 This study
ITS4 TCCTCCGCTTATTGATATGC 52 White et al. (1991)
ITS4_tul CCGCCAGATTCACACATTGA 55 Taylor & McCormick (2008)
ITS4_hormo TCCTCCGCTGAATAATATGC 52.1 This study
LROR ACCCGCTGAACTTAAGC 52 Moncalvo et al. (2000)
LROR_hormo ACCCGCTTGA_TTTAAGC 50 This study
LR3 CCGTGTTTCAAGACGGG 53 Moncalvo et al. (2000)
LR3R GTCTTGAAACACGGACC 50 Moncalvo et al. (2000)
LR5 TCCTGAGGGAAACTTCG 51 Moncalvo et al. (2000)
LR8 CACCTTGGAGACCTGCT 54 Hopple & Vilgalys (1999)